Novel genetic modules encoding high-level antibiotic-free protein expression in probiotic lactobacilli.

ABSTRACT: Lactobacilli are ubiquitous in nature, often beneficially associated with animals as commensals and probiotics, and are extensively used in food fermentation. Due to this close-knit association, there is considerable interest to engineer them for healthcare applications in both humans and animals, for which high-performance and versatile genetic parts are greatly desired. For the first time, we describe two genetic modules in Lactiplantibacillus plantarum that achieve high-level gene expression using plasmids that can be retained without antibiotics, bacteriocins or genomic manipulations. These include (i) a promoter, P_tlpA , from a phylogenetically distant bacterium, Salmonella typhimurium, which drives up to 5-fold higher level of gene expression compared to previously reporte

SUBMITTER: Dey S 

PROVIDER: S-EPMC10221531 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature