Identification of Key Residues in Dengue Virus NS1 Protein That Are Essential for Its Secretion.

ABSTRACT: Dengue virus (DENV) non-structural protein 1 (NS1) is involved in multiple aspects of the DENV lifecycle. Importantly, it is secreted from infected cells as a hexameric lipoparticle that mediates vascular damage that is a hallmark of severe dengue. Although the secretion of NS1 is known to be important in DENV pathogenesis, the exact molecular features of NS1 that are required for its secretion from cells are not fully understood. In this study, we employed random point mutagenesis in the context of an NS1 expression vector encoding a C-terminal HiBiT luminescent peptide tag to identify residues within NS1 that are essential for its secretion. Using this approach, we identified 10 point mutations that corresponded with impaired NS1 secretion, with in silico analyses indicating that the maj

SUBMITTER: Tan BEK 

PROVIDER: S-EPMC10221731 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature