Influence of Ionizing Radiation on Spontaneously Formed Aggregates in Proteins or Enzymes Solutions.

ABSTRACT: We have shown that many proteins and enzymes (ovalbumin, β-lactoglobulin, lysozyme, insulin, histone, papain) undergo concentration-dependent reversible aggregation as a result of the interaction of the studied biomolecules. Moreover, irradiation of those protein or enzyme solutions under oxidative stress conditions results in the formation of stable soluble protein aggregates. We assume that protein dimers are mainly formed. A pulse radiolysis study has been made to investigate the early stages of protein oxidation by N3• or ^•OH radicals. Reactions of the N3• radical with the studied proteins lead to the generation of aggregates stabilized by covalent bonds between tyrosine residues. The high reactivity of the ^•OH with amino acids contained within proteins is respons

SUBMITTER: Radomska K 

PROVIDER: S-EPMC10221975 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature