Targeted Mutations Produce Divergent Characteristics in Pedigreed Sake Yeast Strains.

ABSTRACT: Modification of the genetic background and, in some cases, the introduction of targeted mutations can play a critical role in producing trait characteristics during the breeding of crops, livestock, and microorganisms. However, the question of how similar trait characteristics emerge when the same target mutation is introduced into different genetic backgrounds is unclear. In a previous study, we performed genome editing of AWA1, CAR1, MDE1, and FAS2 on the standard sake yeast strain Kyokai No. 7 to breed a sake yeast with multiple excellent brewing characteristics. By introducing the same targeted mutations into other pedigreed sake yeast strains, such as Kyokai strains No. 6, No. 9, and No. 10, we were able to create sake yeasts with the same excellent brewing

SUBMITTER: Klinkaewboonwong N 

PROVIDER: S-EPMC10222758 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature