Successive Acanthamoeba Corneal Isolates Identified in Poland Monitored in Terms of In Vitro Dynamics.

ABSTRACT:

Background

Amoebae of the genus Acanthamoeba cause a sight-threatening infection called Acanthamoeba keratitis. It is considered a rare disease in humans but poses an increasing threat to public health worldwide, including in Poland. We present successive isolates from serious keratitis preliminary examined in terms of the identification and monitoring of, among others, the in vitro dynamics of the detected strains.

Methods

Clinical and combined laboratory methods were applied; causative agents of the keratitis were identified at the cellular and molecular levels; isolates were cultivated in an axenic liquid medium and regularly monitored.

Results

In a phase-contrast microscope, Acanthamoeba sp. cysts and live trophozoites from corneal samples and

SUBMITTER: Chomicz L 

PROVIDER: S-EPMC10222970 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature