Triggering RNA Interference by Photoreduction under Red Light Irradiation.

ABSTRACT: RNA interference (RNAi) using small interfering RNAs (siRNAs) is a powerful tool to target any protein of interest and is becoming more suitable for in vivo applications due to recent developments in RNA delivery systems. To exploit RNAi for cancer treatment, it is desirable to increase its selectivity, e.g., by a prodrug approach to activate the siRNAs upon external triggering, e.g., by using light. Red light is especially well suited for in vivo applications due to its low toxicity and higher tissue penetration. Known molecular (not nanoparticle-based) red-light-activatable siRNA prodrugs rely on singlet oxygen (¹O₂)-mediated chemistry. ¹O₂ is highly cytotoxic. Additionally, one of the side products in the activation of the known siRNA prodrugs

SUBMITTER: Ruhle J 

PROVIDER: S-EPMC10223155 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature