Development of a Crystal Digital RT-PCR for the Detection of Atypical Porcine Pestivirus.

ABSTRACT: Atypical porcine pestivirus (APPV), a newly discovered virus, is associated with the type A-II congenital tremor (CT) in neonatal piglets. APPV distributes throughout the world and causes certain economic losses to the swine industry. The specific primers and probe were designed targeting the 5' untranslated region (UTR) of APPV to amplify a 90 bp fragment, and the recombinant standard plasmid was constructed. After optimizing the concentrations of primers and probe, annealing temperature, and reaction cycles, a crystal digital RT-PCR (cdRT-PCR) and real-time quantitative RT-PCR (qRT-PCR) were successfully established. The results showed that the standard curves of the qRT-PCR and the cdRT-PCR had R² values of 0.999 and 0.9998, respectively. Both methods could specifically detec

SUBMITTER: Liu H 

PROVIDER: S-EPMC10223196 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature