Immunoaffinity biosensors for the detection of SARS-CoV-1 using screened Fv-antibodies from an autodisplayed Fv-antibody library.

ABSTRACT: The detection of severe acute respiratory syndrome coronavirus (SARS-CoV-1) was demonstrated using screened Fv-antibodies for SPR biosensor and impedance spectrometry. The Fv-antibody library was first prepared on the outer membrane of E. coli using autodisplay technology and the Fv-variants (clones) with a specific affinity toward the SARS-CoV-1 spike protein (SP) were screened using magnetic beads immobilized with the SP. Upon screening the Fv-antibody library, two target Fv-variants (clones) with a specific binding affinity toward the SARS-CoV-1 SP were determined and the Fv-antibodies on two clones were named "Anti-SP1" (with CDR3 amino acid sequence: ¹GRTTG⁵NDRPD¹¹Y) and "Anti-SP2" (with CDR3 amino acid sequence: ¹CLRQA⁵GTADD

SUBMITTER: Jung J 

PROVIDER: S-EPMC10223632 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature