Application of Benchtop NMR for Metabolomics Study Using Feces of Mice with DSS-Induced Colitis.

ABSTRACT: Nuclear magnetic resonance (NMR)-based metabolomics, which comprehensively measures metabolites in biological systems and investigates their response to various perturbations, is widely used in research to identify biomarkers and investigate the pathogenesis of underlying diseases. However, further applications of high-field superconducting NMR for medical purposes and field research are restricted by its high cost and low accessibility. In this study, we applied a low-field, benchtop NMR spectrometer (60 MHz) employing a permanent magnet to characterize the alterations in the metabolic profile of fecal extracts obtained from dextran sodium sulfate (DSS)-induced ulcerative colitis model mice and compared them with the data acquired from high-field NMR (800 MHz). Nineteen metabolites were a

SUBMITTER: Song Z 

PROVIDER: S-EPMC10224185 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature