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Optimization of Carob Products Preparation for Targeted LC-MS/MS Metabolomics Analysis.


ABSTRACT: Carob (Ceratonia siliqua) is an exceptional source of significant bioactive compounds with great economic importance in the Mediterranean region, where it is widely cultivated. Carob fruit is used for the production of a variety of products and commodities such as powder, syrup, coffee, flour, cakes, and beverages. There is growing evidence of the beneficial effects of carob and the products made from it on a range of health problems. Therefore, metabolomics could be used to explore the nutrient-rich compounds of carob. Sample preparation is a crucial step in metabolomics-based analysis and has a great impact on the quality of the data obtained. Herein, sample preparation of carob syrup and powder was optimized, to enable highly efficient metabolomics-based HILIC-MS/MS analysis. Poo

SUBMITTER: Deda O 

PROVIDER: S-EPMC10224476 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature