Deletion of Rap-phosphatases for quorum sensing control in Bacillus and its effect on surfactin production.

ABSTRACT: The complex regulatory network in Bacillus, known as quorum sensing, offers many opportunities to modify bacterial gene expression and hence to control bioprocesses. One target regulated by this mechanism is the activity of the P_srfA promoter, which is engaged in the formation of lipopeptide surfactin. It was hypothesised that deletion of rapC, rapF and rapH, encoding for prominent Rap-phosphatases known to affect P_srfA activity, would enhance surfactin production. Therefore, these genes were deleted in a sfp⁺ derivative of B. subtilis 168 with subsequent evaluation of quantitative data. Up to the maximum product formation of the reference strain B. subtilis KM1016 after 16 h of cultivation, the titers of the rap deletion mutants did not exceed the referenc

SUBMITTER: Treinen C 

PROVIDER: S-EPMC10224909 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature