De novo transcriptome assembly and annotation for gene discovery in Salamandra salamandra at the larval stage.

ABSTRACT: Dispersal is a key process in ecology and evolutionary biology, as it shapes biodiversity patterns over space and time. Attitude to disperse is unevenly distributed among individuals within populations, and that individual personality can have pivotal roles in the shaping of this attitude. Here, we assembled and annotated the first de novo transcriptome of the head tissues of Salamandra salamandra from individuals, representative of distinct behavioral profiles. We obtained 1,153,432,918 reads, which were successfully assembled and annotated. The high-quality of the assembly was confirmed by three assembly validators. The alignment of contigs against the de novo transcriptome led to a mapping percentage higher than 94%. The homology annotation with DIAMOND led to 153,048 (blastx) and 95,94

SUBMITTER: Libro P 

PROVIDER: S-EPMC10224929 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature