Cx3cr1 controls kidney resident macrophage heterogeneity.

ABSTRACT: Kidney macrophages are comprised of both monocyte-derived and tissue resident populations; however, the heterogeneity of kidney macrophages and factors that regulate their heterogeneity are poorly understood. Herein, we performed single cell RNA sequencing (scRNAseq), fate mapping, and parabiosis to define the cellular heterogeneity of kidney macrophages in healthy mice. Our data indicate that healthy mouse kidneys contain four major subsets of monocytes and two major subsets of kidney resident macrophages (KRM) including a population with enriched Ccr2 expression, suggesting monocyte origin. Surprisingly, fate mapping data using the newly developed Ms4a3^Cre Rosa Stop^f/f TdT model indicate that less than 50% of Ccr2⁺ KRM are derived fro

SUBMITTER: Yashchenko A 

PROVIDER: S-EPMC10225589 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature