Oxford nanopore long-read sequencing enables the generation of complete bacterial and plasmid genomes without short-read sequencing.

ABSTRACT:

Introduction

Genome-based analysis is crucial in monitoring antibiotic-resistant bacteria (ARB)and antibiotic-resistance genes (ARGs). Short-read sequencing is typically used to obtain incomplete draft genomes, while long-read sequencing can obtain genomes of multidrug resistance (MDR) plasmids and track the transmission of plasmid-borne antimicrobial resistance genes in bacteria. However, long-read sequencing suffers from low-accuracy base calling, and short-read sequencing is often required to improve genome accuracy. This increases costs and turnaround time.

Methods

In this study, a novel ONT sequencing method is described, which uses the latest ONT chemistry with improved accuracy to assemble genomes of MDR strains and plasmids from long-read sequencing data only. Three

SUBMITTER: Zhao W 

PROVIDER: S-EPMC10225699 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature