Spatial transcriptomics of murine bone marrow megakaryocytes at single-cell resolution.

ABSTRACT:

Background

While megakaryocytes are known for making platelets, recent single-cell RNA sequencing data have revealed subpopulations of megakaryocytes with predicted immunoregulatory and bone marrow niche-supporting roles. Although these studies uncovered interesting information regarding the transcriptional variation of megakaryocytes, the generation, localization, and regulation of these subsets have not yet been studied and therefore remain incompletely understood. Considering the complex organization of the bone marrow, we reasoned that the application of spatial transcriptomic approaches could help dissect megakaryocyte heterogeneity within a spatiotemporal context.

Objectives

The aim of this study was to combine spatial context and transcriptomics to assess the heteroge

SUBMITTER: Tilburg J 

PROVIDER: S-EPMC10225915 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature