Development of Raman Calibration Model Without Culture Data for In-Line Analysis of Metabolites in Cell Culture Media.

ABSTRACT: In this study, we developed a method to build Raman calibration models without culture data for cell culture monitoring. First, Raman spectra were collected and then analyzed for the signals of all the mentioned analytes: glucose, lactate, glutamine, glutamate, ammonia, antibody, viable cells, media, and feed agent. Using these spectral data, the specific peak positions and intensities for each factor were detected. Next, according to the design of the experiment method, samples were prepared by mixing the above-mentioned factors. Raman spectra of these samples were collected and were used to build calibration models. Several combinations of spectral pretreatments and wavenumber regions were compared to optimize the calibration model for cell culture monitoring without culture data. The ac

SUBMITTER: Hara R 

PROVIDER: S-EPMC10225996 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature