Low testosterone state inhibits erectile function by downregulating the expression of GIT1 in rat penile corpus cavernosum

ABSTRACT: Abstract

Background

The mechanism of erectile dysfunction (ED) caused by a low androgen level is still not clear.

Aim

To explore the influence of the low testosterone state on G protein–coupled receptor kinase interactor 1 (GIT1) and its contact to erectile function.

Methods

Thirty male Sprague-Dawley rats aged 8 weeks were distributed at random into 5 groups: control (sham operated), castration, testosterone supplement after castration, castration + vacant lentiviral transfection, and castration + lentiviral transfection. The testis and epididymis were removed through a scrotal incision to develop castrated rats. Four weeks after castration, a lentivirus carrying the GIT1 gene was injected into the middle of rat penile corpus cavernosum. One week after transfect

SUBMITTER:  

PROVIDER: S-EPMC10226814 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature