Strategic Application of Epigenetic Regulators for Efficient Neuronal Reprogramming of Human Fibroblasts.

ABSTRACT:

Background and objectives

Cellular reprogramming in regenerative medicine holds great promise for treating patients with neurological disorders. In this regard, small molecule-mediated cellular conversion has attracted special attention because of its ease of reproducibility, applicability, and fewer safety concerns. However, currently available protocols for the direct conversion of somatic cells to neurons are limited in clinical application due of their complex nature, lengthy process, and low conversion efficiency.

Methods and results

Here, we report a new protocol involving chemical-based direct conversion of human fibroblasts (HF) to matured neuron-like cells with a short duration and high conversion efficiency using temporal and strategic dual epigenetic regulation. I

SUBMITTER: Fernandes GS 

PROVIDER: S-EPMC10226862 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature