Cochlear transcript diversity and its role in auditory functions implied by an otoferlin short isoform.
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ABSTRACT:
SUBMITTER: Liu H
PROVIDER: S-EPMC10227054 | biostudies-literature | 2023 May
REPOSITORIES: biostudies-literature
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Cochlear transcript diversity and its role in auditory functions implied by an otoferlin short isoform.
Liu Huihui H Liu Hongchao H Wang Longhao L Song Lei L Jiang Guixian G Lu Qing Q Yang Tao T Peng Hu H Cai Ruijie R Zhao Xingle X Zhao Ting T Wu Hao H Wu Hao H
Nature communications 20230529 1
Isoforms of a gene may contribute to diverse biological functions. In the cochlea, the repertoire of alternative isoforms remains unexplored. We integrated single-cell short-read and long-read RNA sequencing techniques and identified 236,012 transcripts, 126,612 of which were unannotated in the GENCODE database. Then we analyzed and verified the unannotated transcripts using RNA-seq, RT-PCR, Sanger sequencing, and MS-based proteomics approaches. To illustrate the importance of identifying splice ...[more]
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