Structural insights into the bi-specific cross-over dual variable antibody architecture by cryo-EM.

ABSTRACT: Multi-specific antibodies (msAbs) are being developed as next generation antibody-based therapeutics. Knowledge of the three-dimensional structures, in the full antibody context, of their fragment antigen-binding (Fab) moieties with or without bound antigens is key to elucidating their therapeutic efficiency and stability. However, the flexibility of msAbs, a feature essential for their multi specificity, has hindered efforts in this direction. Cross-Over Dual Variable immunoglobulin (CODV_Ig) is a promising bispecific antibody format, designed to simultaneously target the interleukins IL4 and IL13. In this work we present the biophysical and structural characterisation of a CODV_Fab:IL13 complex in the full antibody context, using cryo-electron microscopy at an overall

SUBMITTER: Fernandez-Martinez D 

PROVIDER: S-EPMC10227088 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature