Technical report: surgical preparation of human brain tissue for clinical and basic research.

ABSTRACT:

Background

The study of the distinct structure and function of the human central nervous system, both in healthy and diseased states, is becoming increasingly significant in the field of neuroscience. Typically, cortical and subcortical tissue is discarded during surgeries for tumors and epilepsy. Yet, there is a strong encouragement to utilize this tissue for clinical and basic research in humans. Here, we describe the technical aspects of the microdissection and immediate handling of viable human cortical access tissue for basic and clinical research, highlighting the measures needed to be taken in the operating room to ensure standardized procedures and optimal experimental results.

Methods

In multiple rounds of experiments (n = 36), we developed and refined surgical prin

SUBMITTER: Straehle J 

PROVIDER: S-EPMC10227129 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature