CpG DNA methylation changes during epididymal sperm maturation in bulls.

ABSTRACT:

Background

During epididymal transit spermatozoa acquire specific morphological features which enhance their ability to swim in a progressive manner and interact with the oocytes. At the same time, sperm cells undergo specific molecular rearrangements essential for the fertilizing sperm to drive a correct embryo development. To assess epigenetic sperm changes during epididymal maturation, the caput, corpus and cauda epididymis sperm tracts were isolated from eight bulls and characterized for different sperm quality parameters and for CpG DNA methylation using Reduced Representation Bisulfite Sequencing (RRBS) able to identify differentially methylated regions (DMRs) in higher CpG density regions.

Results

Caput sperm showed significant variation in motility and sperm kinetics

SUBMITTER: Capra E 

PROVIDER: S-EPMC10228035 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature