Analysis of BRCA2 Copy Number Loss and Genomic Instability in Circulating Tumor Cells from Patients with Metastatic Castration-resistant Prostate Cancer.

ABSTRACT:

Background

BRCA2 alterations predict for a response to poly-ADP-ribose polymerase inhibition in metastatic castration-resistant prostate cancer (mCRPC). However, detection is hindered by insufficient tumor tissue and low sensitivity of cell-free DNA for detecting copy number loss.

Objective

To evaluate the BRCA2 loss detection using single-cell, shallow whole-genome sequencing (sWGS) of circulating tumor cells (CTCs) in patients with mCRPC.

Design, setting, and participants

We analyzed CTC samples collected concurrently with tumor biopsies intended for clinical sequencing in patients with progressing mCRPC.

Outcome measurements and statistical analysis

Differences in proportions were evaluated using the chi-square test. Correlations between assays were analyzed

SUBMITTER: Barnett ES 

PROVIDER: S-EPMC10228632 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature