PLC regulates spontaneous glutamate release triggered by extracellular calcium and readily releasable pool size in neocortical neurons.

ABSTRACT:

Introduction

Dynamic physiological changes in brain extracellular calcium ([Ca²⁺]_o) occur when high levels of neuronal activity lead to substantial Ca²⁺ entry via ion channels reducing local [Ca²⁺]_o. Perturbations of the extracellular microenvironment that increase [Ca²⁺]_o are commonly used to study how [Ca²⁺] regulates neuronal activity. At excitatory synapses, the Ca²⁺-sensing receptor (CaSR) and other G-protein coupled receptors link [Ca²⁺]_o and spontaneous glutamate release. Phospholipase C (PLC) is activated by G-proteins and is hypothesized to mediate this process.

Methods

Patch-clamping

SUBMITTER: Feldthouse MG 

PROVIDER: S-EPMC10228687 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature