RANKL⁺ senescent cells under mechanical stress: a therapeutic target for orthodontic root resorption using senolytics.

ABSTRACT: In dentistry, orthodontic root resorption is a long-lasting issue with no effective treatment strategy, and its mechanisms, especially those related to senescent cells, remain largely unknown. Here, we used an orthodontic intrusion tooth movement model with an L-loop in rats to demonstrate that mechanical stress-induced senescent cells aggravate apical root resorption, which was prevented by administering senolytics (a dasatinib and quercetin cocktail). Our results indicated that cementoblasts and periodontal ligament cells underwent cellular senescence (p21⁺ or p16⁺) and strongly expressed receptor activator of nuclear factor-kappa B (RANKL) from day three, subsequently inducing tartrate-resistant acid phosphatase (TRAP)-positive odontoclasts and provoking apical roo

SUBMITTER: Zhou Y 

PROVIDER: S-EPMC10229558 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature