Rapid digital pathology of H&E-stained fresh human brain specimens as an alternative to frozen biopsy.

ABSTRACT:

Background

Hematoxylin and Eosin (H&E)-based frozen section (FS) pathology is presently the global standard for intraoperative tumor assessment (ITA). Preparation of frozen section is labor intensive, which might consume up-to 30 minutes, and is susceptible to freezing artifacts. An FS-alternative technique is thus necessary, which is sectioning-free, artifact-free, fast, accurate, and reliably deployable without machine learning and/or additional interpretation training.

Methods

We develop a training-free true-H&E Rapid Fresh digital-Pathology (the-RFP) technique which is 4 times faster than the conventional preparation of frozen sections. The-RFP is assisted by a mesoscale Nonlinear Optical Gigascope (mNLOG) platform with a streamlined rapid artifact-compensated 2D large-f

SUBMITTER: Borah BJ 

PROVIDER: S-EPMC10229595 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature