Implications of Protein Interaction in the Speciation of Potential V^IVO-Pyridinone Drugs.

ABSTRACT: Vanadium complexes (VCs) are promising agents for the treatment, among others, of diabetes and cancer. The development of vanadium-based drugs is mainly limited by a scarce knowledge of the active species in the target organs, which is often determined by the interaction of VCs with biological macromolecules like proteins. Here, we have studied the binding of [V^IVO(empp)₂] (where Hempp is 1-methyl-2-ethyl-3-hydroxy-4(1H)-pyridinone), an antidiabetic and anticancer VC, with the model protein hen egg white lysozyme (HEWL) by electrospray ionization-mass spectrometry (ESI-MS), electron paramagnetic resonance (EPR), and X-ray crystallography. ESI-MS and EPR techniques reveal that, in aqueous solution, both the species [V^IVO(empp)₂] and [V

SUBMITTER: Ferraro G 

PROVIDER: S-EPMC10230503 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature