Matrigel-based organoid culture of malignant mesothelioma reproduces cisplatin sensitivity through CTR1.

ABSTRACT: Organoids are a three-dimensional (3D) culture system that simulate actual organs. Therefore, tumor organoids are expected to predict precise response to chemotherapy in patients. However, to date, few studies have studied the drug responses in organoids of malignant mesothelioma (MM). The poor prognosis of MM emphasizes the importance of establishing a protocol for generating MM-organoid for research and clinical use. Here, we established murine MM organoids from p53^+/- or wild-type C57BL/6 strain by intraperitoneal injection either with crocidolite or carbon nanotube. Established MM-organoids proliferated in Matrigel as spheroids. Subcutaneous injection assays revealed that the MM-organoids mimicked actual tissue architecture and maintained the original histological features o

SUBMITTER: Ito F 

PROVIDER: S-EPMC10230733 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature