Analysis of the microglia transcriptome across the human lifespan using single cell RNA sequencing.

ABSTRACT:

Background

Microglia are tissue resident macrophages with a wide range of critically important functions in central nervous system development and homeostasis.

Method

In this study, we aimed to characterize the transcriptional landscape of ex vivo human microglia across different developmental ages using cells derived from pre-natal, pediatric, adolescent, and adult brain samples. We further confirmed our transcriptional observations using ELISA and RNAscope.

Results

We showed that pre-natal microglia have a distinct transcriptional and regulatory signature relative to their post-natal counterparts that includes an upregulation of phagocytic pathways. We confirmed upregulation of CD36, a positive regulator of phagocytosis, in pre-natal samples compared to adult sample

SUBMITTER: Yaqubi M 

PROVIDER: S-EPMC10230780 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature