Amplicon-Based High-Throughput Sequencing Method for Genotypic Characterization of Norovirus in Oysters.

ABSTRACT: Norovirus is a highly diverse RNA virus often implicated in foodborne outbreaks, particularly those associated with shellfish. Shellfish are filter feeders, and when harvested in bays exposed to wastewater overflow or storm overflows, they can harbor various pathogens, including human-pathogenic viruses. The application of Sanger or amplicon-based high-throughput sequencing (HTS) technologies to identify human pathogens in shellfish faces two main challenges: (i) distinguishing multiple genotypes/variants in a single sample and (ii) low concentrations of norovirus RNA. Here, we assessed the performance of a novel norovirus capsid amplicon HTS method. We generated a panel of spiked oysters containing various norovirus concentrations with different genotypic compositions. Several DNA polymer

SUBMITTER: Fitzpatrick AH 

PROVIDER: S-EPMC10231197 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature