Low energy nebulization preserves integrity of SARS-CoV-2 mRNA vaccines for respiratory delivery.

ABSTRACT: Nebulization of mRNA therapeutics can be used to directly target the respiratory tract. A promising prospect is that mucosal administration of lipid nanoparticle (LNP)-based mRNA vaccines may lead to a more efficient protection against respiratory viruses. However, the nebulization process can rupture the LNP vehicles and degrade the mRNA molecules inside. Here we present a novel nebulization method able to preserve substantially the integrity of vaccines, as tested with two SARS-CoV-2 mRNA vaccines. We compare the new method with well-known nebulization methods used for medical respiratory applications. We find that a lower energy level in generating LNP droplets using the new nebulization method helps safeguard the integrity of the LNP and vaccine. By comparing nebulization techniques wi

SUBMITTER: van Rijn CJM 

PROVIDER: S-EPMC10231294 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature