Alzheimer's disease pathogenetic progression is associated with changes in regulated retained introns and editing of circular RNAs.

ABSTRACT:

Introduction

The molecular changes leading to Alzheimer's disease (AD) progression are poorly understood. A decisive factor in the disease occurs when neurofibrillary tangles (NFT) composed of microtubule associated protein tau (MAPT) form in the entorhinal cortex and then spread throughout the brain.

Methods

We therefore determined mRNA and circular RNA changes during AD progression, comparing Braak NFT stages I-VI. Total RNA was isolated from human brain (entorhinal and frontotemporal cortex). Poly(A)+ RNA was subjected to Nanopore sequencing, and total RNA was analyzed by standard Illumina sequencing. Circular RNAs were sequenced from RNase R treated and rRNA depleted total RNA. The sequences were analyzed using different bioinformatic tools, and expression constructs for

SUBMITTER: Arizaca Maquera KA 

PROVIDER: S-EPMC10231643 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature