Establishment of isotype-switched, antigen-specific B cells in multiple mucosal tissues using non-mucosal immunization.

ABSTRACT: Although most pathogens infect the human body via mucosal surfaces, very few injectable vaccines can specifically target immune cells to these tissues where their effector functions would be most desirable. We have previously shown that certain adjuvants can program vaccine-specific helper T cells to migrate to the gut, even when the vaccine is delivered non-mucosally. It is not known whether this is true for antigen-specific B cell responses. Here we show that a single intradermal vaccination with the adjuvant double mutant heat-labile toxin (dmLT) induces a robust endogenous, vaccine-specific, isotype-switched B cell response. When the vaccine was intradermally boosted, we detected non-circulating vaccine-specific B cell responses in the lamina propria of the large intestines, Peyer's pa

SUBMITTER: Prior JT 

PROVIDER: S-EPMC10231862 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature