Dynamics of RNA m⁵C modification during brain development.

ABSTRACT: Post-transcriptional RNA modifications have been recognized as key regulators of neuronal differentiation and synapse development in the mammalian brain. While distinct sets of 5-methylcytosine (m⁵C) modified mRNAs have been detected in neuronal cells and brain tissues, no study has been performed to characterize methylated mRNA profiles in the developing brain. Here, together with regular RNA-seq, we performed transcriptome-wide bisulfite sequencing to compare RNA cytosine methylation patterns in neural stem cells (NSCs), cortical neuronal cultures, and brain tissues at three postnatal stages. Among 501 m⁵C sites identified, approximately 6% are consistently methylated across all five conditions. Compared to m⁵C sites identified in NSCs, 96% of them were h

SUBMITTER: Johnson Z 

PROVIDER: S-EPMC10232014 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature