Glutathione Upregulates the Expression of K_ATP Channels and Vasorelaxation Responses and Inhibits mPTP Opening and Oxidative Stress in the Heart Mitochondria of Old Rats.

ABSTRACT:

Background

In the present work, we investigated the effect of exogenous glutathione in old rats on the expression of ATP-sensitive potassium (K_ATP) channels, the mitochondrial permeability transition pore (mPTP) opening in the heart, and the vasorelaxation responses of isolated aortic rings to activation of K_ATP channels.

Methods

Experiments were performed on adult (6 months) and old (24 months) male Wistar rats, which were divided into three groups: adult, old, and glutathione-treated old rats. Glutathione was injected intraperitoneally at a dose of 52 mg/kg 1 hour before the studies. The mRNA expression of K_ATP channels was determined using reverse transcription and real-time polymerase chain reaction analysis. The effect of glutathione adm

SUBMITTER: Strutynskyi R 

PROVIDER: S-EPMC10232108 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature