A multi-site, international laboratory study to assess the performance of penicillin susceptibility testing of Staphylococcus aureus.

ABSTRACT:

Objectives

There is clinical uncertainty over the optimal treatment for penicillin-susceptible Staphylococcus aureus (PSSA) infections. Furthermore, there is concern that phenotypic penicillin susceptibility testing methods are not reliably able to detect some blaZ-positive S. aureus.

Methods

Nine S. aureus isolates, including six genetically diverse strains harbouring blaZ, were sent in triplicate to 34 participating laboratories from Australia (n = 14), New Zealand (n = 6), Canada (n = 12), Singapore (n = 1) and Israel (n = 1). We used blaZ PCR as the gold standard to assess susceptibility testing performance of CLSI (P10 disc) and EUCAST (P1 disc) methods. Very major errors (VMEs), major error (MEs) and categorical agreement were calculated.

Results

Twenty-two labo

SUBMITTER: Henderson A 

PROVIDER: S-EPMC10232234 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature