Anticancer efficacy of hirsuteine against colorectal cancer by opposite modulation of wild-type and mutant p53.

ABSTRACT:

Purpose

The present study aimed to explore the anticancer activity of hirsuteine (HST), an indole alkaloid from the traditional Chinese herbal medicine Uncaria rhynchophylla, against colorectal cancer (CRC) and the underlining mechanism.

Methods

MTT, colony formation, flow cytometry and MDC staining were conducted to confirm the antiproliferative effect of HST on human CRC cells harboring different p53 status. Protein expressions were evaluated by the Western blot analysis. p53 protein half-life and the interaction between p53 and MDM2 were investigated using cycloheximide (CHX)-chase assay and Co-immunoprecipitation (Co-IP), respectively. Transcriptional activity of p53 was examined by qRT-PCR and Chromatin immunoprecipitation (ChIP). Xenograft tumor in nude mice was create

SUBMITTER: Zhang Y 

PROVIDER: S-EPMC10232392 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature