Next-generation RNA sequencing elucidates transcriptomic signatures of pathophysiologic nerve regeneration.

ABSTRACT: The cellular and molecular underpinnings of Wallerian degeneration have been robustly explored in laboratory models of successful nerve regeneration. In contrast, there is limited interrogation of failed regeneration, which is the challenge facing clinical practice. Specifically, we lack insight on the pathophysiologic mechanisms that lead to the formation of neuromas-in-continuity (NIC). To address this knowledge gap, we have developed and validated a novel basic science model of rapid-stretch nerve injury, which provides a biofidelic injury with NIC development and incomplete neurologic recovery. In this study, we applied next-generation RNA sequencing to elucidate the temporal transcriptional landscape of pathophysiologic nerve regeneration. To corroborate genetic analysis, nerves were

SUBMITTER: Warner WS 

PROVIDER: S-EPMC10232541 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature