Whole genome sequencing of Mycobacterium bovis directly from clinical tissue samples without culture.

ABSTRACT: Advancement in next generation sequencing offers the possibility of routine use of whole genome sequencing (WGS) for Mycobacterium bovis (M. bovis) genomes in clinical reference laboratories. To date, the M. bovis genome could only be sequenced if the mycobacteria were cultured from tissue. This requirement for culture has been due to the overwhelmingly large amount of host DNA present when DNA is prepared directly from a granuloma. To overcome this formidable hurdle, we evaluated the usefulness of an RNA-based targeted enrichment method to sequence M. bovis DNA directly from tissue samples without culture. Initial spiking experiments for method development were established by spiking DNA extracted from tissue samples with serially diluted M. bovis BCG DN

SUBMITTER: Zeineldin M 

PROVIDER: S-EPMC10232834 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature