Elevated, FcεRI-dependent MRGPRX2 expression on basophils in chronic urticaria.

ABSTRACT:

Background

Chronic urticaria (CU) is a skin condition driven by mast cells and basophils. The exact responsiveness profile of these cells, especially regarding the anti-IgE treatment, Omalizumab, is not fully investigated. We sought to characterize the surface activation profile of basophils in CU during Omalizumab treatment and their responsiveness to IgE and non-IgE stimulation.

Methods

Whole blood basophils from 11 CU patients and 10 healthy controls were stimulated with either medium, anti-IgE, fMLP, C5a, or Substance P for 30 min and characterized by flow cytometry.

Results

CU patients showed a broad range of basophil count as opposed to healthy subjects. An increased number of unstimulated CD69⁺ (p = 0.05), but not CD63⁺ basophils wa

SUBMITTER: Bartko EA 

PROVIDER: S-EPMC10233071 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature