The role of nitrogen-responsive regulators in controlling inorganic polyphosphate synthesis in Escherichia coli.

ABSTRACT: Inorganic polyphosphate (polyP) is synthesized by bacteria under stressful environmental conditions and acts by a variety of mechanisms to promote cell survival. While the kinase that synthesizes polyP (PPK, encoded by the ppk gene) is well known, ppk transcription is not activated by environmental stress and little is understood about how environmental stress signals lead to polyP accumulation. Previous work has shown that the transcriptional regulators DksA, RpoN (σ⁵⁴) and RpoE (σ²⁴) positively regulate polyP production, but not ppk transcription, in Escherichia coli. In this work, we examine the role of the alternative sigma factor RpoN and nitrogen starvation stress response pathways in controlling polyP synthesis. We show that the RpoN

SUBMITTER: Bowlin MQ 

PROVIDER: S-EPMC10233264 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature