Silibinin, an HSP90 Inhibitor, on Human ACTH-Secreting Adenomas.

ABSTRACT:

Introduction

The glucocorticoid receptor is pivotal to control corticotrophin (ACTH) secretion, and its function is closely linked to the heat shock protein 90 (HSP90) chaperone complex. Impaired sensitivity to glucocorticoid feedback is a hallmark of human corticotroph adenomas, i.e., Cushing's disease, a disorder with few medical treatment options. Silibinin, a HSP90 inhibitor, has been studied in tumoral corticotroph cells and its use proposed in Cushing's disease. Aim of the present study was to further investigate the effect of silibinin on human corticotroph adenomas in vitro.

Methods

Seven human ACTH-secreting pituitary adenomas were established in culture and treated with 10-50 µm silibinin with/without dexamethasone for up to 72 h. ACTH medium levels were m

SUBMITTER: Pecori Giraldi F 

PROVIDER: S-EPMC10233703 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature