Interleukin-33 increases type 2 innate lymphoid cell count and their activation in eosinophilic asthma.

ABSTRACT:

Background

Interleukin-33 (IL-33) exacerbates asthma probably through type 2 innate lymphoid cells (ILC2s). Nevertheless, the association between eosinophilic asthma (EA) and ILC2s remains obscure, and the mechanisms by which IL-33 affects ILC2s are yet to be clarified.

Methods

ILC2s were evaluated in peripheral blood mononuclear cells, induced sputum, and bronchoalveolar lavage fluid obtained from patients with EA. Confocal microscopy was performed to locate ILC2s in lung tissue and the mRNA expression of ILC2-related genes was also evaluated in the EA model. The proliferation of ILC2s isolated from humans and mice was assessed following IL-33 or anti-IL-33 stimulation.

Results

The counts, activation, and mRNA expression of relevant genes in ILC2s were higher in PBMC

SUBMITTER: Sun F 

PROVIDER: S-EPMC10234174 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature