LncNALT knockdown ameliorates hypertensive retinopathy via PTEN/PI3K/AKT pathway.

ABSTRACT: This study aimed to explore the role of the long non-coding RNA NOTCH1-associated lncRNA in T cell acute lymphoblastic leukemia (lncNALT) in the pathogenesis of hypertensive retinopathy (HR). LncNALT expression levels were determined using reverse transcription-quantitative polymerase chain reaction. The effects of lncNALT knockdown on the viability, proliferation, migration, and invasion of human retinal microvascular endothelial cells (RMECs) were determined via 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide, 5-ethynyl-2'-deoxyuridine staining, and Transwell assays. Protein expression levels were determined using western blotting. We found that lncNALT expression levels were increased in RMECs treated with hydrogen peroxide (H₂O₂), while the knockdown

SUBMITTER: Kang H 

PROVIDER: S-EPMC10234215 | biostudies-literature | 2022 Jul-Dec

REPOSITORIES: biostudies-literature