Chromatin structure influences rate and spectrum of spontaneous mutations in Neurospora crassa.

ABSTRACT: Although mutation rates have been extensively studied, variation in mutation rates throughout the genome is poorly understood. To understand patterns of genetic variation, it is important to understand how mutation rates vary. Chromatin modifications may be an important factor in determining variation in mutation rates in eukaryotic genomes. To study variation in mutation rates, we performed a mutation accumulation (MA) experiment in the filamentous fungus Neurospora crassa and sequenced the genomes of the 40 MA lines that had been propagated asexually for approximately 1015 [Formula: see text] mitoses. We detected 1322 mutations in total and observed that the mutation rate was higher in regions of low GC, in domains of H3K9 trimethylation, in centromeric regions, and in domains of

SUBMITTER: Villalba de la Pena M 

PROVIDER: S-EPMC10234303 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature