A high-throughput amplicon sequencing approach for population-wide species diversity and composition survey.

ABSTRACT: Management of agricultural pests requires an understanding of pest species diversity, their interactions with beneficial insects and spatial-temporal patterns of pest abundance. Invasive and agriculturally important insect pests can build up very high populations, especially in cropping landscapes. Traditionally, sampling effort for species identification involves small sample sizes and is labour intensive. Here, we describe a multiprimer high throughput sequencing (HTS) metabarcoding method and associated analytical workflow for a rapid, intensive, high-volume survey of pest species compositions. We demonstrate our method using the taxonomically challenging Bemisia pest cryptic species complex as examples. The whiteflies Bemisia including the"tabaci" species are agriculturally important c

SUBMITTER: Tay WT 

PROVIDER: S-EPMC10234416 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature