Systems biology elucidates the distinctive metabolic niche filled by the human gut microbe Eggerthella lenta.

ABSTRACT: Human gut bacteria perform diverse metabolic functions with consequences for host health. The prevalent and disease-linked Actinobacterium Eggerthella lenta performs several unusual chemical transformations, but it does not metabolize sugars and its core growth strategy remains unclear. To obtain a comprehensive view of the metabolic network of E. lenta, we generated several complementary resources: defined culture media, metabolomics profiles of strain isolates, and a curated genome-scale metabolic reconstruction. Stable isotope-resolved metabolomics revealed that E. lenta uses acetate as a key carbon source while catabolizing arginine to generate ATP, traits which could be recapitulated in silico by our updated metabolic model. We compared these in vitro findings with metabolite shifts o

SUBMITTER: Noecker C 

PROVIDER: S-EPMC10234575 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature