Reconstruction of the personal information from human genome reads in gut metagenome sequencing data.

ABSTRACT: Human DNA present in faecal samples can result in a small number of human reads in gut shotgun metagenomic sequencing data. However, it is presently unclear how much personal information can be reconstructed from such reads, and this has not been quantitatively evaluated. Such a quantitative evaluation is necessary to clarify the ethical concerns related to data sharing and to enable efficient use of human genetic information in stool samples, such as for research and forensics. Here we used genomic approaches to reconstruct personal information from the faecal metagenomes of 343 Japanese individuals with associated human genotype data. Genetic sex could be accurately predicted based on the sequencing depth of sex chromosomes for 97.3% of the samples. Individuals could be re-identified fro

SUBMITTER: Tomofuji Y 

PROVIDER: S-EPMC10234815 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature