Influence of class 2 integron integrase concentration on gene cassette insertion and excision in vivo.

ABSTRACT: Integron can capture and express antimicrobial resistance gene cassettes and plays important roles in horizontal gene transfer. The establishment of a complete in vitro reaction system will help to reveal integron integrase mediated site-specific recombination process and regulation mechanism. As an enzymatic reaction, the concentration of integrase is assumed to have a great influence on the reaction rate. To determine the influence of different concentrations of integrase on the reaction rate and to find the best range of enzyme concentration were essential to optimizing the in vitro reaction system. In this study, plasmids with gradient transcription levels of class 2 integron integrase gene intI2 under different promoters were constructed. Among plasmids pI2W16, pINTI2N, pI2W, and pI2N

SUBMITTER: Zhu Y 

PROVIDER: S-EPMC10235263 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature