M-MDSC in vitro generation from mouse bone marrow with IL-3 reveals high expression and functional activity of arginase 1.

ABSTRACT: Myeloid-derived suppressor cells (MDSC) represent major regulators of immune responses, which can control T cells via their inducible nitric oxide synthase (iNOS)- and arginase 1 (Arg1)-mediated effector functions. While GM-CSF is well documented to promote MDSC development, little is known about this potential of IL-3, an established growth factor for mast cells. Here, we show that IL-3, similar to GM-CSF, generates monocytic MDSC (M-MDSC) from murine bone marrow (BM) cells after 3 days of in vitro culture. At this time point, predominantly CD11b⁺ CD49a⁺ monocytic and CD11b⁺ CD49a^- FcεR I^- neutrophilic cells were detectable, while CD11b^low/neg FcεR I⁺ mast cells accumulated only after extended cultu

SUBMITTER: Aintablian A 

PROVIDER: S-EPMC10235456 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature